The author attempted to observe the cellose acetate microgone electrophoretic patterns,quantitative estimation, and histcctemical localization of alkaline fhcsphatase from the
sera and various organ tissues. in rabbit.In the electrophoretic analysis the tissue ALP from the liver (α1and α2, lung (α2/β,β, and, β/r), bone (α2, α2/β, and β), kidney (α2, α2/β, β/r and γ),
Iymph node (α1, α2, α3/β, and β), spleen
(α2/β and β), and intestine (α2/β and β) showed the
fractions of the high activity, while the heart and skeletal muscle 6id not show the any
fraction of ALP.
In the quantitative measurements of tissue ALP activity per gram unit extract the
kidney ALP showed the highest level (49.5 Bessey-Lowly units), and the activity of the
skeletal muscle ALP was the lowest (1.5 B.L. units).
From the histochemical localization the tissue ALP was confirmed mainly in the
biliary canaliculi of the liver, the convoluted tubules of the kidney, the intestinal and
bladder mucosa, the bronchial and bronctiolar epithelium and the alveoli of the lung,
white pulps of the spleen, the cortex and medulla of the adrenal gland, the lymph node
and the brain tissue respectively, while there was no ALP activity in the heart and the
skeletal muscle.
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